Nerve growth factor (NGF) is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons. Source: DNA sequence encoding the human beta NGF protein sequence (containing the signal peptide, pro-peptide and the mature beta NGF sequence) was expressed in modified human 293 cells. Theoretical Peptide Sequence: YAEHKSHRGEYSVCDSESLWVTDKSSAIDIRGHQVTVLGEIKTGNSPVKQYFYETRCKEAR PVKNGCRGIDDKHWNSQCKTSQTYVRALTSENNKLVGWRWIRIDTSCVCALSRKIGRT Biological. Activity: ED50 of beta NGF is typically 0.2-1.0 ng/ml as measured in a cell proliferation assay using the human growth factor dependent TF-1 cell line. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: The NGF protein migrates at ~12-16kD in SDS-PAGE. It has a predicted molecular mass of 13.5kD. The protein separates into a number of isoforms with a pI between 9 and 10 in 2D PAGE due to post-translational modifications. The unmodified protein has a predicted pI of 9. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
