Neurite Outgrowth Inhibitor Protein B, Rat (Nogo-B) (Control Peptide)
Biozol Catalog Number:
USB-N2116-41
Supplier Catalog Number:
N2116-41
Alternative Catalog Number:
USB-N2116-41-100
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
ELISA
Control peptide for N2116-42 (affinity purified antibody) and N2116-43 (antiserum). Many tissues such as muscle, skin, liver, and peripheral nerve, have remarkable ability to repair and regrowafter injury. The CNS (brain and spinal cord) is limited in its ability to repair or regrowth causing permanent brain damage or paralyses. An inhibitory myelin protein, Nogo (Neurite outgrowth), has been cloned and characterized. It may help block the regeneration of the CNS. Nogo is the 4th member of reticulon (Rtn) family. There are three alternative isoforms of Nogo. Nogo-A is full length human protein consisting of 1192aa, Mr ~135kD (rat 1163aa). Nogo-B is an intermediate form consistiong of 373aa, ~37kD. Nogo-B lacks aa186-1004 within the extracellular domain. Nogo-C is shorter consisting of 199aa, ~25kD. It lacks aa186-1004 and has a smaller, alternative N-terminal domain. It is similar to rat vp20 and foocen-s. Nogo-A has a putative extracellular domain of 1024aa, 2-3 TM domains, and a short C-terminus of 43aa. Nogo-A is localized to the CNS-myelin. It is highly expressed in oligodendorcytes, but not by Schwann cells. Nogo-B and Nogo-C have been found in several non-neuronal tissues (skeletal muscle, kidney, skin, lung, and spleen). Applications: Suitable for use in ELISA, Antibody Blocking. Not suitable for use in Western Blot due to low molecular weight. Other applications not tested. Recommended Dilution: Antibody Blocking: 5-10ug per 1ug N2116-42 (affinity purified antibody) or 1ul N2116-43 (antiserum). ELISA: 50-100ng of control peptide/well. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Highly purified
Form:
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide
* VAT and and shipping costs not included. Errors and price changes excepted
