Neurofilaments can be defined as the intermediate or 10nm filaments found specifically in neuronal cells. In the electron microscope neurofilaments appear as 10nm diameter fibers of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. They probably function to provide structural support for neurons and their synapses and to support the large axon diameters required for rapid conduction of impulses down axons. They are composed a mixture of subunits which usually include the three neurofilament triplet proteins known as NFL, NFM and NFH. Neurofilaments may also include smaller amounts of peripherin, alpha internexin, nestin and in some cases vimentin. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable. Form: Supplied as a lyophilized powder from 10mM sodium phosphate, pH 7.5, 2mM DTT, 6M Urea, 1mM EDTA, 10mM methylammonium chloride. Reconstitute with sterile ddH2O to a concentration of ~1mg/ml. Purity: 98% (determined by SDS gel electrophoresis). Isoelectric Point: pI 5.5 Applications: Suitable for use as a Protein standard in 1D and 2D SDS gel electrophoresis, Immunoassays and for Immunization. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
200
Purity:
98% (determined by SDS gel electrophoresis).
Form:
Supplied as a lyophilized powder from 10mM sodium phosphate, pH 7.5, 2mM DTT, 6M Urea, 1mM EDTA, 10mM methylammonium chloride. Reconstitute with sterile ddH2O to a concentration of ~1mg/ml.
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