Applications: Tested in ELISA and immunoblotting with anti-NSE antibodies. Activity: 93umol/min/mg at 25C Assay Buffer: 45 mM imidazole, pH 6.8, 45 mM potassium chloride, 1.33 mM magnesium sulfate, 1mM 2-PG. Unit Definition: One unit is defined as the amount of NSE converted 1mol of 2-phospho-D-glycerate/minute at 25C. Sub-unit composition is gamma-gamma.
Purity:
95% (SDS-PAGE) Purified by ion-exchange chromatography and gel-filtration.
Form:
Supplied as a liquid in 0.1M Tris HCl, 5mM magnesium sulfate, 0.25M potassium chloride, pH 5.1.
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