NTHL1, Recombinant, Human, aa1-312, His-Tag (Endonuclease III-like protein 1, NTH1, OCTS3, EC 4.2.99.18)

Product Overview

• Article Name: NTHL1, Recombinant, Human, aa1-312, His-Tag (Endonuclease III-like protein 1, NTH1, OCTS3, EC 4.2.99.18)
• Biozol Catalog Number: USB-N2291-43
• Supplier Catalog Number: N2291-43
• Alternative Catalog Number: USB-N2291-43-50,USB-N2291-43-250
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

NTHL1 has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5 from the damaged site. Required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions Recombinant protein corresponding to aa1-312 from human NTHL1 protein fused to His-tag at the N-terminus, expressed in E. coli Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MCSPQESGMT ALSARMLTRS RSLGPGAGPR GCREEPGPLR RREAAAEARK SHSPVKRPRK AQRLRVAYEG SDSEKGEGAE PLKVPVWEPQ DWQQQLVNIR AMRNKKDAPV DHLGTEHCYD SSAPPKVRRY QVLLSLMLSS QTKDQVTAGA MQRLRARGLT VDSILQTDDA TLGKLIYPVG FWRSKVKYIK QTSAILQQHY GGDIPASVAE LVALPGVGPK MAHLAMAVAW GTVSGIAVDT HVHRIANRLR WTKKATKSPE ETRAALEEWL PRELWHEING LLVGFGQQTC LPVHPRCHAC LNQALCPAAQ GL Molecular Weight: 36.6kD (332aa) confirmed by MALDI-TOF Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 36.6
• NCBI: 002519
• UniProt: P78549
• Purity: 80% (SDS-PAGE)
• Form: Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 0.15M sodium chloride, 1mM DTT, 40% glycerol