Peptide is used to block NF-kappaB p65 Rabbit mAb reactivity in immunohistochemistry protocols. Transcription factors of the nuclear factor kappaB (NF-kappaB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-kappaB1 (p105/p50) and NF-kappaB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. The p50 and p52 products form dimeric complexes with Rel proteins, which are then able to bind DNA and regulate transcription. In unstimulated cells, NF-kappaB is sequestered in the cytoplasm by its inhibitory proteins, the IkappaBs (3-5). NF-kappaB-activating agents can induce the phosphorylation of IkappaBs, targeting them for rapid degradation through an ubiquitin-proteasome pathway, releasing NF-kappaB to enter the nucleus, where it regulates gene expression (6-8). Processing of NF-kappaB2 p100 is regulated by NIK and IKK1 (IKKalpha), which triggers the phosphorylation and processing to p52, which can then undergo nuclear translocation (9-11). Quality Control: The quality of the peptide was evaluated by reversed-phase HPLC and by mass spectrometry. The peptide blocks NF-kappaB p65 Rabbit mAb signal in immunohistochemistry. Directions For Use: For immunohistochemistry, add twice the volume of peptide as volume of antibody used in 100ul total volume. Incubate for a minimum of 30 minutes prior to adding the entire volume to the slide. Recommended antibody dilutions can be found on the product data sheet. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Highly Purified
Form:
Supplied as a liquid in 20 mM potassium phosphate (pH 7.0), 50 mM NaCl, 0.1 mM EDTA, 1 mg/ml BSA and 5% glycerol.
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