NFKBIA, Recombinant, Human, aa1-317, His-Tag (NF-kappa-B Inhibitor alpha, I-kappa-B-alpha, IkappaBalpha, IkB-alpha, Major Histocompatibility Complex Enhancer-binding Protein MAD3, NFKBI, MAD3, IKBA)
Biozol Catalog Number:
USB-N2306-61A
Supplier Catalog Number:
N2306-61A
Alternative Catalog Number:
USB-N2306-61A-50,USB-N2306-61A-250
Manufacturer:
US Biological
Category:
Molekularbiologie
NF-kappa-B inhibitor alpha, also known as NFKBIA, is members of the IkB family of proteins can be divided into four groups (IkB-alpha, IkB-beta, IkB-gamma, IkB-epsilon). NFKBIA inhibits the NFkB complex by binding and sequestering it in the cytoplasm. Upon stimulation, NFKBIA is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Recombinant corresponding to aa1-317 from human NFKBIA, fused to His-tag at N-terminal expressed in E.coli. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MFQAAERPQE WAMEGPRDGL KKERLLDDRH DSGLDSMKDE EYEQMVKELQ EIRLEPQEVP RGSEPWKQQL TEDGDSFLHL AIIHEEKALT MEVIRQVKGD LAFLNFQNNL QQTPLHLAVI TNQPEIAEAL LGAGCDPELR DFRGNTPLHL ACEQGCLASV GVLTQSCTTP HLHSILKATN YNGHTCLHLA SIHGYLGIVE LLVSLGADVN AQEPCNGRTA LHLAVDLQNP DLVSLLLKCG ADVNRVTYQG YSPYQLTWGR PSTRIQQQLG QLTLENLQML PESEDEESYD TESEFTEFTE DELPYDDCVF GGQRLTL Molecular Weight: ~37.7kD (MALDI-TOF) Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
