Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitrotyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimers disease, Parkinsons disease and amyotrophic lateral sclerosis (4). Applications: Suitable for use in Western Blot, ELISA, Flow Cytometry, Immunoprecipitation, Immunohistochemistry and Immunofluorescence/Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: 1:100 Western Blot: 1:1400 0.7ug/ml detects 5ug SIN-1 treated BSA using Goat anti-mouse IgG-HRP as the secondary antibody. Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[7L25]
Isotype:
IgG2a
Purity:
Purified by Protein G affinity chromatography.
Form:
Supplied as a liquid in PBS, pH 7.4, 0.09% azide, 50% glycerol.
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