Nitrotyrosine has been identified as an indicator of cell damage and inflammation, as well as of the production of NO. It is believed that measuring the concentration of nitrotyrosine will serve as a marker for damage caused by NO in the cell. Previous studies have shown that nitrotyrosine has been found in inflammatory conditions such as atherosclerotic plaques and rheumatoid arthritis, among others. Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml detects nitrated proteins in a lysate prepared from peroxynitrite A431 cells. A previous lot detected nitrotyrosine immunoblotting controls. A previous lot detected nitrated proteins in a lysate prepared from peroxynitrite-treated A431 cells. No reaction was noted with non-nitrated proteins. Immunocytochemistry: 5-10ug/ml showed positive immunostaining for nitrated proteins in peroxynitrite-treated (5-10mM for 5 min) A431 cells fixed with 95% ethanol/5% acetic acid. Optimal dilutions to be determined by the researcher. Recommended Controls: Immunohistochemistry: Human Alzheimer diease brain sections (Methacarn fixed), Mouse livers Western Blot: Nitrated-BSA Indirect ELISA: Nitrated-BSA Indirect Immunofluorescence: A549 cells metabolically labeled with 3-nitrosine Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Clonality:
Monoclonal
Clone Designation:
[0.T.98 1A6]
Purity:
Purified by Protein G affinity chromatography.
Form:
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
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