A natural cytotoxicity receptor (NCR) NKp46 has been shown to represent a novel NK cell-specific molecule involved in human NK cell activation. The natural cytotoxicity receptors (NCRs) are a recently characterized family of Ig-like activation receptors that appear to be major triggering receptors in tumor cell recognition. The three known NCRs include NKp46 and NKp30, which are expressed on circulating NK cells, and NKp44, which is expressed only on activating NK cells. NKp46 has been implicated in NK cell-mediated lysis of several autologous tumor cells and pathogen-infected cell lines. NKp46 has two extracellular Ig-like domains followed by a ~40 residue stalk region, a type I transmembrane domain, and a short cytoplasmic tail. The extracellular Ig-like domain of NKp46 (aa22-255) was overexpressed in E. coli. Molecular Weight: 26.6kD (235 amino acids) Sequence: MQQQTLPKPF/ IWAEPHFMVP/ KEKQVTICCQ/ GNYGAVEYQL/ HFEGSLFAVD/ RPKPPERINK/ VKFYIPDMNS/ RMAGQYSCIY/ RVGELWSEPS/ NLLDLVVTEM/ YDTPTLSVHP/ GPEVISGEEV/ TFYCRLDTAT/ SMFLLLKEGR/ SSHVQRGYGK/ VQAEFPLGPV/ TTAHRGTYRX/ FGSYNNHAWS/ FPSEPVKLLV/ TGDIENTSLA/ PEDPTFSADT/ WGTYLLTTET/ GLQKDHALWD/ HTAQN Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
26.6
Purity:
95% by SDS PAGE, Purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer.
Form:
Supplied as a liquid in PBS, pH 7.4, 1mM EDTA.
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