Organic Anion Transporter 1, Control Peptide, Rat (OAT1)
Biozol Catalog Number:
USB-O3003-03
Supplier Catalog Number:
O3003-03
Alternative Catalog Number:
USB-O3003-03-100
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
ELISA
Control Peptide for O3003-01 (antiserum) and O3003-02 (affinity purified) A 16aa Peptide sequence near the cytoplasmic, C-terminus of rat OAT1. Mammalian kidney and liver are critical in maintaining physiological ionic environment. Kidney specializes in removing toxins, drugs, and other organic anions from the blood by a process called renal secretion. Besides kidney, anionic substrates are also transported in other organs, e.g., choroid plexus, eye, airway, and placenta. Several multispecific OATs (OAT1-3, OAT-K1 and OATK2) and OATPs (organic anion transporting polypeptides, oatp1-3), have been cloned and characterized from various tissues. OATPs family of proteins are very similar in sequence and secondary protein structure (up to 12 transmembrane domains with cytoplasmic N and C-terminus). Rat Kidney PAH transporter, termed OAT1 or ROAT1, encodes a protein of 551 aa. Human OAT1 (hOAT1) is 563 aa and its alternatively spliced isoform hOAT2 is 550 aa (missing 13 aa from 523-533 aa). OAT1 has wide substrate selectivity, covering endogenous substrates such as cyclic nucleotides, prostaglandin and uric acid, and a variety of drugs (e.g., antibiotics, non-steroidal anti-inflammatory drugs, diuretics, anti-neoplastic drug, and a uricosuric drug). OAT1 has been localized on the basolateral membrane of the proximal tubule in the kidney. Weak expression was also detected in brain. OAT1 has ~95 identity with mouse NKT, an ortholog of rat OAT1. Applications: Suitable for use in ELISA and Antibody Blocking. Other applications not tested. Recommended Dilution: ELISA: 1ug/ml Antibody Blocking: 5-10ug/1ul O3003-01 or 1ug O3003-02 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Highly purified
Form:
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
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