Obestatin, Control Peptide (Appetite-regulating Hormone, Growth Hormone Secretagogue, Growth Hormone-releasing Peptide, Motilin-related Peptide, Protein M46, Ghrelin, GHRL, MTLRP)
Biozol Catalog Number:
USB-O4802-21B
Supplier Catalog Number:
O4802-21B
Alternative Catalog Number:
USB-O4802-21B-100
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
ELISA
Control peptide for O4802-21 (affinity purified) and O4802-21A (antiserum). Control of appetite involves a number of gastrointestinal hormones that belong to the ghrelin-motilin ligand family that either stimulate food intake and gastric emptying or inhibit these responses. Recently, a new member of the ghrelin-motilin family was identified by using bioinformatic predictions about enzyme cleavage of the prepropeptide of ghrelin. The newly identified 23aa ghrelin-associated peptide was named obestatin. Although both peptides originate from the same precursor prepropeptide, they have opposing physiological roles. Obestatin exerts anorexigenic effects by decreasing food intake, gastric emptying, jejunal motility, and weight gain by binding to GPR39 orphan receptor, while ghrelin exerts opposing orexigenic effects by binding to growth hormone secretagogue receptor. Obestatin: Human, mouse, rat 23aa each - 2.6kD, mouse Chromosome: 6E3. Mainly expressed in the gastrointestinal tract with higher levels in the stomach, medium levels in the duodenum, jejunum, ileum and colon. Low expression in the testis and brain. Not detected in the salivary gland, pancreas, liver and lung. Source: Synthetic peptide corresponding to 23aa of mouse Obestatin (Accession Q9EQX0), N-terminal, extracellular. Molecular Weight: ~3kD Applications: Suitable for use in ELISA and Antibody Blocking. Not suitable for Western Blot. Other applications not tested. Recommended Dilution: ELISA: 5-10ug control peptide per 1ug of aff pure IgG or 1ul antiserum Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
