POU5F1 (POU domain, class 5, Transcription factor 1, OCT4, DADB-104B20.2, POU class 5 homeobox 1, Octamer-binding transcription factor-3, POU-type Homeodomain-containing DNA-binding protein, OTF3, OCT3, MGC22487, HGNC:9221, POU do
Biozol Catalog Number:
USB-O5403-01Y
Supplier Catalog Number:
O5403-01Y
Alternative Catalog Number:
USB-O5403-01Y-100
Manufacturer:
US Biological
Host:
Goat
Category:
Antikörper
Application:
ELISA, WB
Immunogen:
Synthetic peptide corresponding to C-VTTLGSPMHSN, from human POU5F1, at C-terminal (NP_002692.2, NP_976034.3). Species sequence homology: Canine, rat and mouse.
Transcription factor that binds to the octamer motif (5-ATTTGCAT-3). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:16,000 Western Blot: 1-3ug/ml, observed in human liver lysates on ~38kd bands Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
