Oligo, Counterclockwise Sequencing Primer, pBR322 (Hind III), (16-mer), [5-GCA ATT TAA CTG TGA T-3]

Product Overview

• Article Name: Oligo, Counterclockwise Sequencing Primer, pBR322 (Hind III), (16-mer), [5-GCA ATT TAA CTG TGA T-3]
• Biozol Catalog Number: USB-O6020-33
• Supplier Catalog Number: O6020-33
• Alternative Catalog Number: USB-O6020-33-33
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Single-stranded oligonucleotide, chemically synthesized, suitable for DNA applications. This sequencing primer is used to sequence DNA that has been cloned into pBR322. Special Labels: Biotin, Fluorescein, and Phosphate modifications can be included at the 5 or 3 site. For special labels add -B, -F, -P and -5, -3 to the catalog number. Sequence: 5 GCA ATT TAA CTG TGA T 3 Molecular Weight: 4895.2g/mol GC Content: 31.2% Tm: 41.5C Synthesis Scale: 50nmole Synthesis scale refers to the amount of the 3-terminus base that the multi-step synthesis starts with. Final yield is indicated on the lot-specific certificate of analysis. Minimum Guaranteed Yield: 1.0 OD (A260nm) unit per mL reconstituted product 1 OD unit corresponds to ~33ug ssDNA Storage & Stability: Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Purity: Purified by HPLC. Quality Control: MALDI-TOF MS
• Form: Supplied as a lyophilized powder. Reconstitute with sterile dH2O, TE or Tris buffer, pH 7.0.