Osteoprotegerin, Recombinant, Human (OPG, Osteoclastogenesis Inhibitory Factor, OCIF)

Product Overview

• Article Name: Osteoprotegerin, Recombinant, Human (OPG, Osteoclastogenesis Inhibitory Factor, OCIF)
• Biozol Catalog Number: USB-O8065-01
• Supplier Catalog Number: O8065-01
• Alternative Catalog Number: USB-O8065-01-10,USB-O8065-01-50
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Osteoprotegerin (OPG) is a member of the TNFR superfamily that can act as a decoy receptor for RANKL. Binding of soluble OPG to sRANKL inhibits osteoclastogenesis by interrupting the signaling between stromal cells and osteoclastic progenitor cells, thereby leading to excess accumulation of bone and cartilage. OPG is expressed in a wide variety of tissues, including the adult heart, lung, kidney, liver, spleen, prostate, lymph node, and bone marrow. OPG is secreted both as a monomeric and a dimeric protein. Its primary structure consists of seven distinct domains, four of which correspond to the extracellular cysteine-rich domains of TNFR proteins and constitute the soluble OPG. Recombinant Human OPG is a soluble 20kD protein containing 174 amino acid residues. Amino Acid Sequence: METFPPKYLH YDEETSHQLL CDKCPPGTYL KQHCTAKWKT VCAPCPDHYY TDSWHTSDEC LYCSPVCKEL QYVKQECNRT HNRVCECKEG RYLEIEFCLK HRSCPPGFGV VQAGTPERNT VCKRCPDGFF SNETSSKAPC RKHTNCSVFG LLLTQKGNAT HDNICSGNSE STQK Biological Activity: Determined by its ability to inhibit TRAIL-induced apoptosis of LN-18 glioblastoma cells. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile Tris, pH 7.5. For long term storage add a carrier protein (0.1% BSA). Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• UniProt: O00300
• Purity: 98% (SDS-PAGE and HPLC). Endotoxin: 0.1ng/ug (1EU/ug).
• Form: Supplied as a lyophilized powder from 10mM Tris, pH7.5, 75mM sodium chloride. Reconstitute with 5mM Tris, pH 7.5, to a concentration of 0.1-1mg/ml. Do not vortex. For long term storage add a carrier protein (0.1% BSA).