Many antibodies are made to small peptides or haptens that are injected as Ovalbumin-conjugates. Antibodies are produced against the carrier protein (Ovalbumin) and the coupled peptide or hapten. Anti-Ovalbumin antibodies may interfere with some analyses. The Ovalbumin-agarose column can be used to remove the anti- Ovalbumin antibodies using standard antibody purification techniques. Applications: Suitable for use in removing anti-ovalbumin antibodies in antisera. Other applications not tested. Usage: We recommend processing approximately 1-5ml antiserum per 1ml of the beads or it can be scaled up accordingly. Load antiserum diluted 1:5 in PBS to adsorb anti-Ovalbumin at room temperature. Collect unbound fraction containing Ovalbumin-depleted antiserum. It may be necessary to repeat this adsorption if the sample contains high concentrations of ant-Ovalbumin. Regeneration: The column can be regenerated by passing 3ml of 0.1M Glycine buffer, pH 2.5, and then immediately washing with with 10-20 volumes of PBS, pH 7.4. Store column in PBS containing 0.05% azide at 4C. DO NOT FREEZE the beads at any stage. Storage and Stability: Stored at 4C. Do not freeze.
Molecular Weight:
43000
Form:
The affinity matrix is supplied in PBS pH 7.4 containing 0.05% azide. The column has a binding capacity of ~2-10mg anti-Ovalbumin per ml of beads. Typically, this may corresponds to 5-10ml antiserum containing antibodies to Ovalbumin. The anti-Ovalbumin I
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