5-T C A T G A-3 3-A G T A C T-5 Concentration: 10u/ul Incubation Temperature: 37C Units for Overnight Incubation: 0.2 Thermal Inactivation: 80C Ligation and Recleavage: After 50-fold overdigestion with PagI, more than 90% of the DNA fragments can be ligated and recut. Number of Recognition Sites in DNA Molecules: Lambda: 8 PhiX174: 3 M13mp18/19: 1 pBR322: 4 pUC18/19: 3 pUC57: 3 pTZ19R/U: 2 pBluescriptIIKS(-/+): 2 pBluescriptIISK(-/+): 2 pACYC177: 4 pACYC184: 1
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