Paired Immunoglobulin-Like Type 2 Receptor alpha, Recombinant, Mouse (PILR-alpha, PILRa)
Biozol Catalog Number:
USB-P2150-48
Supplier Catalog Number:
P2150-48
Alternative Catalog Number:
USB-P2150-48-50
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
WB
PILR-alpha (paired immunoglobulin-like type 2 receptor-alpha, also FDF03) is one of two members of a small family of immunoregulatory Ig-superfamily receptors (1, 2). It is a counterpart to PILR-Beta and it likely gave rise to PILR-Beta through gene duplication and rearrangement (1). The PILRs represent one of many pairs of Ig-like domain-containing receptors that participate in immune regulation. PILR-alpha and -Beta should not be confused with the similarly named PIRs (also paired immunoglobulin-like receptors ), or the functionally-related SIRP and ILT/LILR/CD85/LIR family of receptors (2). While PIRs, ILTs and SIRPs contain three to six Ig-like domains in their extracellular region, PILR-alpha and -Beta show only one Ig-like region in their extracellular domain (ECD) (1, 2). Mouse PILR-alpha is a monomeric, 271 amino acid (aa) type I transmembrane (TM) protein (3). It contains a 167 aa ECD, a 21 aa TM segment, and a long, 83 aa cytoplasmic region. The ECD shows one V-type Ig-like domain between aa 39-157, while the cytoplasmic region contains two ITIMs (immunoreceptor Tyr-based inhibitory motifs) between aas 265-270 and 294-299. Given that ITIMs are known to interact with phosphatases such as PTPN6 and PTPN11, the presence of these motifs makes mouse PILR-alpha an inhibitory receptor. In human, activation of PILR-alpha inhibits CD32/FcgammaRII-induced calcium mobilization (3). Although CD99 is a known ligand for both PILR-alpha and -Beta (4), highest affinity binding seems to occur between CD99 and PILR-alpha (4). Mouse PILR-alpha is found on neutrophils and macrophages (4). Mouse PILR-alpha ECD is 43% and 69% aa identical to human and rat PILR-alpha ECD, respectively, it is 75% aa identical to the ECD of mouse PILR-Beta (3). One Potential isoform of PILR-alpha has been reported. It varies only within the first 28 aa of the signal sequence (5). Source: A DNA sequence encoding the extracellular domain of mouse PILR-alpha (Leu 21-Val 197, Accession NP_705730) was fused to a 6x histidine tag at the C-terminus. The protein was expressed in a mouse myeloma cell line, NS0. Definition: Measured by its ability to bind rmCD99/mFc. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 20.5kD migrates as an ~ 40-50kD protein in SDS-PAGE under reducing conditions. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
