Parainfluenza virus 2 is one of four serotypes of parainfluenza viruses in the paramoxyvirus family. Parainfluenza viruses are the second most common cause of lower respiratory tract infections in young children. Parainfluenza virus 2 causes upper and lower respiratory tract illnesses and croup. Parainfluenza virus 1, VP1 strain, cultured in Vero cells. Optimally infected monolayers are harvested, disrupted by sonication and subjected to low speed centrifugation. The clarified cell lysate is pooled with supernatant from the infected culture and concentrated using crossflow ultrafiltration with a 30,000 molecular cutoff. The antigen preparation is inactivated using gamma radiation. Source: Parainfluenza virus 2, Greer strain, cultured in Vero cells. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Purified by crossflow ultrafiltration.
Form:
Supplied as a liquid in Tissue Culture Media.
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