Parathyroid Hormone Related Peptide (Parathyroid Hormone-related Protein, PTHrP, PTH-rP, Humoral Hypercalcemia of Malignancy, HHM, MGC14611, Osteostatin, Parathyroid Hormone-like Hormone, PTHLH, Parathyroid Hormone-like Protein, P
Biozol Catalog Number:
USB-P3109-36E
Supplier Catalog Number:
P3109-36E
Alternative Catalog Number:
USB-P3109-36E-100
Manufacturer:
US Biological
Host:
Mouse
Category:
Antikörper
Application:
IHC
Immunogen:
Recombinant protein corresponding to Ala37-Arg175 from human PTHLH expressed in E. coli.
The parathyroid hormone-like hormone (PTHLH) has been recognized in patients suffering from tumors associated with the common neoplastic syndrome humoral hypercalcemia of malignancy (HHM). The 18kD protein is a 177aa precursor that belongs to the parathyroid hormone family. It contains a 24aa signal sequence followed by a 10aa propeptide aa25-34. The mature chain runs from aa37-177. Of note, residues 108-129 constitute a nuclear localization signal (NLS) providing evidence that the protein not only acts as a secreted factor, but in an intracrine manner as well. A second isoform is missing aa176-177, and a 34aa substitution for aa176-177 produces a third isoform. There is a strong homology displayed between PTHLH and human parathyroid hormone (PTH) among the first 13aa. Studies show that the similarity in this area is 67% at the aa and nucleotide levels. Indeed, eight of the first 13aa of PTHLH and PTH are identical. Human PTHLH and mouse PTHLH share 86aa sequence identity. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 8-25ug/ml using immersion fixed paraffin-embedded sections of human colon Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
