PCAF, Active, Recombinant, aa352-832 (p300/CBP-associated factor, CREBBP-associated factor, CAF, P/CAF)

Product Overview

• Article Name: PCAF, Active, Recombinant, aa352-832 (p300/CBP-associated factor, CREBBP-associated factor, CAF, P/CAF)
• Biozol Catalog Number: USB-P3114
• Supplier Catalog Number: P3114
• Alternative Catalog Number: USB-P3114-50
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

A direct link exists between hyperacetylation of chromatin and transcriptional activation. Experiments have established that PCAF possesses intrinsic histone acetylase activity. PCAF primarily acetylates lysine 14 of H3. It acetylates lysine 8 of H4 less efficiently. PCAF also acetylates p53 in response to DNA damage. The distinct patterns of acetylation by PCAF may contribute to transcriptional regulation of important genes. Source: Recombinant GST fusion protein corresponding to aa352-832, expressed in E. coli. Quality Control: HAT Assay: 500ng of PCAF was used to acetylate histones in vitro with tracer levels of [3H]AcetylCoA as the acetyl donor. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 79
• NCBI: 003884
• UniProt: Q92831
• Purity: 30%
• Form: Supplied as a liquid 50mM Tris-HCl, pH 8.0, 150mM sodium chloride, 25mM glutathione, 50% glycerol.