PDK1, active, Recombinant, Human (PDPK1, PI-P3 Dependent Kinase, 3a Phosphoinositide dependent Protein Kinase 1)

Product Overview

• Article Name: PDK1, active, Recombinant, Human (PDPK1, PI-P3 Dependent Kinase, 3a Phosphoinositide dependent Protein Kinase 1)
• Biozol Catalog Number: USB-P3123-01C
• Supplier Catalog Number: P3123-01C
• Alternative Catalog Number: USB-P3123-01C-10
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Recombinant full-length house PDK1 was expressed by baculovirus in Sf9 insect cells using a N-terminal His tag. The gene accession number is NM_002613. Specific Activity: As Reported Assay Conditions: Assay was done in Kinase buffer containing 0.2mM DTT using PDKtide substrate (0.1mg/ml) and 20uM ATP. Reaction was done at 30C for 45 min. Amount of ATP transferred was measured using Kinase-Glo reagent. Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Properties

• Molecular Weight: 67
• NCBI: 002613
• UniProt: O15530
• Purity: ~70%
• Form: Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM KCl, 0.04% Tween 20, 3mM DTT, 20% glycerol.