PHAP III, CT, Control Peptide (Putative HLA DR-associated Protein)
Biozol Catalog Number:
USB-P3368-04A
Supplier Catalog Number:
P3368-04A
Alternative Catalog Number:
USB-P3368-04A-50
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
ELISA, WB
Synthetic peptide corresponding to amino acids close to C-terminus of human PHAP III (1). Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis (1). PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras and Myc-mediated cell transformation. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: Blocks antibody activity completely. Incubate the peptide with equal volume of antibody for 30 min at 37C. Optimal dilutions to be determined by the researcher. Corresponding Antibody: P3368-04-Detects PHAP III. No crossreaction to PHAP I and PHAP I2a. Species Crossreactivity: Human, mouse and rat. Positive Control: A549 or HepG2 cell lysate Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified
Form:
Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.02% sodium azide.
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