After iontophoretic injection of PHA-L, the approximate rate of anterograde transport is about 4-6mM/day, with survival periods of over 18 days having been observed. Once transported, the PHA-L is best visualized for light microscopy with antibody to the lectin, followed by an appropriate ABC kit and DAB. Fluorescent staining can also be used for a faster staining procedure. Source: Red kidney bean Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Homogeneous by SDS-PAGE
Form:
Supplied as a lyophilized powder.
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