Alkaline phosphatase is a broad term associated with non-specific phosphomonesterases with activity optima at alkaline pH. Activity: 0.9 units per mg dry weight (25C, pH 8.8) Unit Definition: 1 unit hydrolyzes 1umole of o-carboxyphenyl phosphate per minute at 25C, pH 8.8. Composition: The enzyme is a zinc metallo-enzyme. Schüssler (1968) reports four isozymes. Chang and Moog (1972) found three isozymes in the enzyme from chicken duodenum (This preparation is from the whole intestine.) Optimum pH: 8-9 A280 mg/ml: As Reported Activators: Schüssler (1968) indicates activation by Mg2+. See Sivanaesan et al. (1991). Inhibitors: Acidification to pH 4.5 reversibly inactivates the enzyme. Quality Control: SDS-PAGE Storage and Stability: Lyophilized powder may be stored at 4C. Stable for 6 months after receipt at 4C.
Purity:
Partially purified
Form:
Supplied as a lyophilized powder
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