Phosphodiesterase 11A4, Recombinant, Human, GST-Tag (PDE11A4)

Product Overview

• Article Name: Phosphodiesterase 11A4, Recombinant, Human, GST-Tag (PDE11A4)
• Biozol Catalog Number: USB-P4072-11D
• Supplier Catalog Number: P4072-11D
• Alternative Catalog Number: USB-P4072-11D-10
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Full length recombinant corresponding to human PDE11A4 with an N-terminal GST tag, expressed in a Baculovirus infected Sf9 cell expression system. Genebank: BC112393. Specific Activity: As reported Unit Definition: 1 unit is the amount of P4072-11D that will convert 1 pmole 3, 5-cAMP to 5-AMP per minute at 37C. Assay Conditions: 10mM Tris-HCl, pH 7.4, 10mM MgCl2, 0.1mg/ml BSA, 0.05% Tween-20, 200uM cAMP and serial dilutions of PDE11A4 at 37C for 20 min. Quantified by 5 nucleotidase cleaving the 5-AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Avoid repeated freezing and thawing. Store at -70C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Properties

• Molecular Weight: 131
• NCBI: 112393
• UniProt: Q9HCR9
• Purity: 90% by SDS-PAGE
• Form: Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM KCl, 0.04% Tween-20, 20% glycerol, 1.6mM glutathione and 3mM DTT.