Phosphodiesterase 1A, Recombinant, Human, GST-Tag (PDE1A)

Product Overview

• Article Name: Phosphodiesterase 1A, Recombinant, Human, GST-Tag (PDE1A)
• Biozol Catalog Number: USB-P4072A
• Supplier Catalog Number: P4072A
• Alternative Catalog Number: USB-P4072A-10
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Human PDE1A (GenBank Accession No. NM_005019), full length with N-terminal GST tag, expressed in a Baculovirus infected Sf9 cell expression system. Specific Activity: 123 U/ug, 1 unit will lease 1nmole phosphate per minute from 400uM 5- AMP at 30C in a reaction buffer of 10mM Tris-HCl, pH 7.4, 0.2mM MgCl2. Assay Buffer: 10mM Tris-HCl, pH 7.4, 0.2mM MgCl2, 0.2mM CaCl2, and 240nM Calmodulin, 3, 5-cAMP substrate: 0.4mM, PDE1A: 1ng/ul. Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

Product Properties

• Molecular Weight: 88.3
• NCBI: 005019
• UniProt: P54750
• Purity: 30% by SDS-PAGE
• Form: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM NaCl, 0.05% Tween 20, 50% glycerol, 20mM glutathione and 3mM DTT.