Phosphodiesterase PDE1B, Recombinant, Human, GST-Tag

Product Overview

• Article Name: Phosphodiesterase PDE1B, Recombinant, Human, GST-Tag
• Biozol Catalog Number: USB-P4072B
• Supplier Catalog Number: P4072B
• Alternative Catalog Number: USB-P4072B-10
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Human PDE1B (GenBank Accession No. NM_000924), full length with N-terminal GST tag. Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Specific Activity: ~400U/ug. One unit is defined as the amount of enzyme that will convert 1pmole of 3, 5-cAMP to 5 AMP per min at 30C in a reaction buffer containing 10mM Tris-HCl, pH 7.4, 0.2mM MgCl2. Assay buffer: 10mM tRIS-HCl, pH 7.4, 0.2mM MgCl2, 0.02mM CaCl2, 240nM Calmodulin, 35-cAMP substrtae: 0.4mM, PDE1B: 2ng/ul. Storage and Stability: Aliquot to avoid repeated freezing and thawing and freeze at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

Product Properties

• Molecular Weight: 87
• NCBI: 000924
• UniProt: Q01064
• Purity: 50% by SDS-PAGE
• Form: Supplied as a liquid in 25mM Tris-HCl, pH 8.0, 100mM sodium chloride, 0.05% Tween 20, 20mM glutathione, 3mM DTT, 50% glycerol.