Phosphoinositide 3-Kinase A, Regulatory Subunit, Recombinant, Bovine
Biozol Catalog Number:
USB-P4073-26C3
Supplier Catalog Number:
P4073-26C3
Alternative Catalog Number:
USB-P4073-26C3-10,USB-P4073-26C3-20
Manufacturer:
US Biological
Category:
Molekularbiologie
Phosphoinositide 3 Kinase, Recombinant, Bovine, p85 alpha (PI3K) functions as the regulatory subunit of the class IA PI3-kinase isoforms a, b, and d. It contains two SH2 domains that bind to tyrosine-phosphorylated growth factor receptors or substrate adaptor proteins. It also contains a BH (breakpoint cluster region homology) domain that shows GAP activity towards the small GTPases Rab4, Rab5, Cdc42, Rac1 and to a lesser extend towards Rab6 and Rab11. It was shown that PI3Ka catalytic subunit mediated phosphorylation of the p85a adapter reduces the lipid kinase activity of the heterodimer and this gives hints for PI3K-dependent signaling events not requiring production of 3-phosphorylated phosphoinositides. PI3Ka protein kinase activity has been implicated in IRS-1 serine phosphorylation in insulin-treated adipocytes and in STAT3 and IRS-1 phosphorylation upon activation of the type 1 IFN receptor by IFN-a. Recombinant bovine Phosphoinositide 3-kinase subunit p85a has a molecular weight of 83.5kD. Storage & Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
