Phosphoinositide 3 Kinase, p85 alpha, p110 beta, Recombinant, Human (PI3K)
Biozol Catalog Number:
USB-P4073-29Z
Supplier Catalog Number:
P4073-29Z
Alternative Catalog Number:
USB-P4073-29Z-2.5,USB-P4073-29Z-5
Manufacturer:
US Biological
Category:
Molekularbiologie
The PI3Kb isoform can be activated by insulin via the insulin receptor to initiate a cascade of events that control cell growth and metabolism. The activation of PI3Kb is mediated by the p85 regulatory subunit binding to tyrosine phosphorylated insulin receptor substrate (IRS) proteins (e.g. IRS-1 and IRS-2). It was also shown that PI3Kb is involved in apoptosis in human colon carcinoma cells. Injection of neutralizing antibodies specific to p110b in WiDr, HCT116 and CO 115 adenocarcinoma cells inhibited de novo DNA synthesis. PI3Kb is the major PI3K isoform required for apoptotic cell and Fc-g receptor mediated phagocytosis shown for primary mouse macrophages and the Jurkat human leukemia T cell line. It was shown by several research groups that the catalytic subunit of PI3Kb can be activated by Gbg subunits of G-protein coupled receptors. Recombinant Human Phosphoinositide 3-kinase beta is a glycosylated protein having a molecular weight as follows: p85 chain 83.5kD, p110 chain 124.3kD. Specific Activity: 3,000 units/mg Unit Definition: 1 unit is defined as 1 picomole phosphate transferred to PIP2/minute Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
8583.51101243
Purity:
90% (SDS-PAGE)
Form:
Supplied as a liquid in PBS and 2mM MgCl2.
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