PIPES is a biological buffer (zwitterionic) designed by Good, et al., and typically referred to as Goods buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240-700nm, chemically stable, and stable to temperature and concentration changes. PIPES has pKa (6.76 at 25C) near the physiological pH which makes it useful in cell culture work. Synonyms: 1,4-Piperazinediethanesulfonic Acid, 1,4-Piperazinebis(ethanesulfonic acid), NSC 157117, Piperazine-1,4-bis(2-ethanesulfonic Acid), Piperazine-N,N-bis(2-ethanesulfonic Acid) CAS No: 5625-37-6 Molecular Formula: C8H18N2O6S2 Molecular Weight: 302.37 Appearance: White powder Purity: 99% Melting Point: >300C (lit) Water (KF): 0.5% Absorbance (A280): 0.1 Absorbance (A260): 0.1 Solubility: 1M NaOH: clear, colorless, complete DNase: None Detected RNase: None Detected Protease: None Detected Storage and Stability: May be stored at RT Stable for 12 months after receipt.
Molecular Weight:
302.37
CAS Number:
[5625-37-6]
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