Plasminogen Activator Inhibitor Type 1, Human, Recombinant (PAI 1)
Catalog Number:
USB-P4256-29
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| Article Name: | Plasminogen Activator Inhibitor Type 1, Human, Recombinant (PAI 1) |
| Biozol Catalog Number: | USB-P4256-29 |
| Supplier Catalog Number: | P4256-29 |
| Alternative Catalog Number: | USB-P4256-29-50 |
| Manufacturer: | US Biological |
| Category: | Molekularbiologie |
| The cardiovascular risk factor plasminogen activator inhibitor type 1 (PAI 1) has been associated with abdominal obesity, hypertension, hypertriglyceridemia, hyperinsulinemia, glucose intolerance, and type II diabetes, conditions known to be linked with insulin resistance. Plasminogen activator inhibitor 1 (PAI 1) is a member of the serine protease inhibitor (serpin) superfamily and a central regulatory protein in the blood coagulation system. PAI-1 is unique among serpins in exhibiting distinct active and inactive (latent) conformations in vivo. Human PAI 1 is a single-chain glycoprotein with a molecular weight of 43kD. This inhibitor acts as ''bait for tissue-type and urokinase-type plasminogen activators (tPA and uPA) and protein C. Its rapid interaction with tPA may function as a major control point in the regulation of fibrinolysis. The highly mobile reactive-center loop (RCL) is thought to account for both the rapid inhibiton of plasminogen activators, and the rapid and spontaneous transition of the unstable, active form of PAI 1 into the stable, inactive conformation. The inactive form can be partially reactivated by denaturants such as urea, guanidine hydrochloride or SDS. High concentrations of PAI 1 have been associated with human thromboembolic disease. PAI 1 activity may limit the extent of tumor metastasis, since uPA activity is a major contributory factor promoting dissolution of tumor matrix and basement membrane. The sequence corresponding to aa24 -427 of human PAI-1 (mature form) tagged with 6xHis on the carboxy-terminal was expressed in E. coli. By uPA activity assay, the recombinant PAI-1 contains a mixture of the active and latent forms, with 45% of active form. The recombinant PAI-1 is able to bind to uPA resulting in a SDS-stable complex. Activity: To fully activate the latent PAI-1, the PAI-1 must be incubated with Activation Buffer (2X Activation Buffer: 8 M Guanidine HCl, 40 mM Sodium Acetate, pH 5.6, 400 mM sodium chloride, 0.2% Tween 20). The recombinant PAI-1 is able to bind to uPA resulting in a SDS-stable complex. Storage and Stability: Aliquot to avoid repeated freezing and thawing and freeze at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for 6 months after receipt. |
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