Plasminogen Activator Inhibitor Type 1, active, Human, Assay Kit, BioAssay(TM) (PAI-1)

Product Overview

• Article Name: Plasminogen Activator Inhibitor Type 1, active, Human, Assay Kit, BioAssay(TM) (PAI-1)
• Biozol Catalog Number: USB-P4256-32
• Supplier Catalog Number: P4256-32
• Alternative Catalog Number: USB-P4256-32-1
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

Plasminogen Activator Inhibitor 1 (PAI-1) is a glycoprotein found in plasma that is implicated in the processes of angiogenesis, hemostasis, tumor metastasis, fibrionlysis, ovulation, embryonic development and inflammation (1). The elevation of PAI-1 has shown to correlate with the prognosis of many cancers including breast cancer (2,3,4). PAI-1 is activated by the glycoprotein vitronectin. Vitronectin binds to PAI-1 and modulates fibrinolysis and cell migration (5). This binding has proven to be an important inhibitor of the activated form of protein C (APC) (6). Principle of the Assay: This is an ELISA for the quantitative analysis of PAI-1 levels in biological fluid. This test kit operates on the basis of sandwich ELISA where active PAI-1 complexes with uPA and is quantified with the use of an HRP-labeled secondary antibody. The functional or active PAI-1 binds to the uPA coated on the well of the microtiter plate. The latent and complexed forms of PAI-1 will not bind and are discarded at a later washing step. Next, a PAI-1 primary antibody is added to the wells binding to the captured PAI-1 on the microtiter plate. HRP-conjugated secondary antibody is then added for detection of the active-PAI-1. Quantitative test results are obtained by the measure and comparison of the sample and standard absorbance readings. Kit Components: P4256-32A: PAI-1 Activity Standard (0u/ml): 1x1ml P4256-32B: PAI-1 Activity Standard (150-350u/ml): 1x1ml P4256-32C: TMB Substrate: 1x10ml P4256-32D: PAI-1 Mab: 1x1 vial P4256-32E: Secondary Antibody (HRP): 1x1 vial P4256-32F: Wash Buffer (10X): 1x50ml P4256-32G: ELISA Buffer: 1x10ml P4256-32H: Primary Antibody Buffer: 1x10ml P4256-32J: Secondary Antibody Buffer: 1x15ml P4256-32K: Microtiter Plate: 1x96 wells Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.