Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)

Product Overview

• Article Name: Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)
• Biozol Catalog Number: USB-P4258
• Supplier Catalog Number: P4258
• Alternative Catalog Number: USB-P4258-2,USB-P4258-10
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

PDGFs are disulfide-linked dimers consisting of two 12.0-13.5kD polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs, PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. Recombinant human PDGF-AA is a 28.5kD disulfide-linked homodimer of two A chains (250aa), expressed in E. coli. Biological Activity: Determined by the dose-dependent stimulation of thymidine uptake by Balb/c 3T3 cells. The expected ED50 for this effect is 1-3ng/ml Amino Acid Sequence: SIEEAVPAVC KTRTVIYEIP RSQVDPTSAN FLIWPPCVEV KRCTGCCNTS SVKCQPSRVH HRSVKVAKVE YVRKKPKLKE VQVRLEEHLE CACATTSLNP DYREEDTGRP RESGKKRKRK RLKPT Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Properties

• Molecular Weight: 28.5
• Purity: 98% by SDS-PAGE and HPLC. Endotoxin: 0.1ng/ug (1EU/ug).
• Form: Supplied as a lyophilized powder. Reconstitute with sterile ddH2O to a 0.1-1mg/ml. Do not vortex. This solution can then be diluted into other aqueous solutions. For extended storage further dilute in a carrier protein.