Platelet Derived Growth Factor BB, Recombinant, Human (PDGF-BB)
Biozol Catalog Number:
USB-P4258-15F
Supplier Catalog Number:
P4258-15F
Alternative Catalog Number:
USB-P4258-15F-10
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
ELISA
Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer. Recombinant protein corresponding to human PDGF-BB, a disulfide-linked homodimer of two B chains, 218 total amino acids, expressed in E. coli. Molecular Weight: ~24.3kD Biological Activity: The ED50 as determined by the dose-dependent stimulation of BALB/c 3T3 cells proliferation is < 1ng/ml Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot and store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.