Platelet Derived Growth Factor CC, Recombinant, Human (PDGF-CC)
Biozol Catalog Number:
USB-P4258-18H
Supplier Catalog Number:
P4258-18H
Alternative Catalog Number:
USB-P4258-18H-5,USB-P4258-18H-20
Manufacturer:
US Biological
Category:
Molekularbiologie
The platelet-derived growth factor (PDGF) family of heparin-binding growth factors consists of five known members, denoted PDGF-AA, PDGF-BB, PDGF-AB, PDGF-CC and PDGF-DD. The mature and active form of these proteins, an anti-parallel disulfide-linked dimer of two 12-14kD polypeptide chains, is obtained through proteolytic processing of biologically inactive precursor proteins, which contain an N-terminal CUB domain and a PDGF/VEGF homologous domain. The PDGFs interact with two related protein tyrosine kinase receptors, PDGFR-alpha and PDGFR-beta, and are potent mitogens for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and certain tumor cells. They play an important role in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubules epithelial cell development. Mature PDGFs are stored in platelet alpha-granules and are released upon platelet activation. PDGF-AA, -AB, -BB and -CC signal primarily through the PDGF-Ralpha receptor, whereas PDGF-DD interacts almost exclusively with the PDGF-Rbeta receptor. Recombinant human PDGF-CC is a 25kD protein consisting of two identical disulfide-linked 114 amino-acid polypeptide chains. Amino Acid Sequence: MVVDLNLLTE EVRLYSCTPR NFSVSIREEL KRTDTIFWPG CLLVKRCGGN CACCLHNCNE CQCVPSKVTK KYHEVLQLRP KTGVRGLHKS LTDVALEHHE ECDCVCRGST GG Biological Activity: Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected The ED50 for this effect is 15-20ng/ml. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Country of Origin: USA
