PPCDC, Recombinant, Human (Phosphopantothenoylcysteine Decarboxylase, PPC-DC, CoaC, MDS018, UNQ9365/PRO34154)
Biozol Catalog Number:
USB-P5505-90
Supplier Catalog Number:
P5505-90
Alternative Catalog Number:
USB-P5505-90-100,USB-P5505-90-500
Manufacturer:
US Biological
Category:
Molekularbiologie
PPCDC belongs to the HFCD (homo oligomeric flavin containing Cys decarboxylase) superfamily that plays a role in the biosynthesis of coenzyme A (CoA) from pantothenate (Vitamin B). The biosynthesis of CoA from pantothenate requires several steps: the phosphorylation of pantothenate, the conversion of 4-hosphopantothenate to 4-phosphopantetheine, the adenylation by phosphopantetheine adenylyltransferase to form dephospho-CoA and the phosphorylation by dephospho-CoA kinase to form CoA. PPCDC plays a direct role in this pathway by converting 4-phosphopantothenate into 4-phosphopantetheine. Source: Recombinant corresponding to aa1-204 of human PPCDC protein, fused to His-tag at N-terminus, expressed in E.coli. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MEPKASCPAA APLMERKFHV LVGVTGSVAA LKLPLLVSKL LDIPGLEVAV VTTERAKHFY SPQDIPVTLY SDADEWEMWK SRSDPVLHID LRRWADLLLV APLDANTLGK VASGICDNLL TCVMRAWDRS KPLLFCPAMN TAMWEHPITA QQVDQLKAFG YVEIPCVAKK LVCGDEGLGA MAEVGTIVDK VKEVLFQHSG FQQS Molecular Weight: 24.6 kD (224aa) confirmed by MALDI-TOF Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
