PRL-1, aa2-173, catalytic domain, Recombinant, Human (Protein tyrosine phosphatase, Phosphatase of regenerating liver-1)
Biozol Catalog Number:
USB-P9003-68
Supplier Catalog Number:
P9003-68
Alternative Catalog Number:
USB-P9003-68-50
Manufacturer:
US Biological
Category:
Molekularbiologie
Catalytic domain, (a.a. 1-173), MW=19.7kD, expressed in an E.coli expression system. The protein encoded by this gene belongs to a small class of prenylated protein tyrosine phosphatases (PTPs), which contains a PTP domain and a characteristic C-terminal prenylation motif. PTPs are cell signaling molecules that play regulatory roles in a variety of cellular processes. This tyrosine phosphatase is a nuclear protein, but may primarily associate with plasma membrane. The surface membrane association of this protein depends on its C-terminal prenylation. Overexpression of this gene in mammalian cells conferred a transformed phenotype, which implicated its role in the tumorigenesis. Studies in rat suggested that this gene may be an immediate-early gene in mitogen-stimulated cells. Application: useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors. Specific Activity: 0.45 U/ug. One unit will hydrolyze 1 pmol 6, 8-difluoro-4-methylumbelliferyl phosphate (DiFMUP) per minute at pH 6.3 and 30C Assay buffer: 50 mM Bis-Tris, pH 6.3, 2 mM EDTA, 2 mM DTT, 100 mM DiFMUP. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
