Protease, S. aureus V8 (Endoproteinase Glu-C), CAS [[66676-43-5]]
Biozol Catalog Number:
USB-P9075
Supplier Catalog Number:
P9075
Alternative Catalog Number:
USB-P9075-1,USB-P9075-5
Manufacturer:
US Biological
Category:
Molekularbiologie
Endoproteinase-Glu-C Protease from S. aureus V8 specifically cleaves peptide bonds on the COOH-terminal side of either aspartic or glutamic acid. In the presence of ammonium buffers, the enzyme specificity is limited to glutamic sites. It has a molecular weight of 27,000 daltons and optimum pHs of 4.0 and 7.8 with hemoglobin as the substrate. It is is inhibited by diisopropylfluorophosphate and monovalent anions such as F-, Cl-, CH3COO- and NO3-. Enzyme activity is determined by the casein digestion assay described by Drapeau (Methods Enzymol., 45, 469, 1976). Activity: 500 units per mg dry weight Absorbance (A280) at 1mg/ml: As reported Unit Definition: One Unit causes a change of 0.001 absorbance unit (at 280nm) per minute at 37C, pH 7.8 using casein as the substrate. Extinction Coefficient: 4.26 Optimum pH: 4.0 and 7.8 with hemoglobin substrate Inhibitors: Diisopropyl fluorophosphate (DFP) and monovalent anions such as F-, Cl-, Br-, CH3COO-, and NO3- Quality Control: SDS-PAGE Storage and Stability: Stable for 6 months after receipt at 4C. Autolysis occurs at temperatures greater than 40C. The enzyme is fully active in 2mg/ml SDS.
Molecular Weight:
27
Purity:
Chromatographically purified
Form:
Supplied as a lyophilized powder.
CAS Number:
[66676-43-5]
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