Heparin Beads are made by coupling 20-35kD size Heparin extracted from Porcine intestinal mucosa (Type I) to Sepharose beads. These beads are useful for isolating heparin-binding protein such as Antithrombin III, lipoproteins, various enzymes and DNA binding proteins. Heparin Beads Specifications: Matrix: Sepharose CL-4B Coupling Method: TargetLock Heparin Density: 0.7-1.2mg/ml of 20-35kD Heparin. Thrombin binding capacity minimum 0.7mg/ml of beads Mean Bead Size: 40 -165um Range of pH: 3.0-11.0 Bead Structure: Highly cross-linked spherical agarose, 4% Max Back Pressure: 0.3MPa, 3 bar Max. Flow Rates: 4ml/min/cm2 Recommended Flow Rate: 1-3ml/min/cm2 Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Form:
Supplied as a liquid in PBS pH 7.4, 0.3N sodium chloride added with 0.01% thimerosal as a preservative.
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