Activated KLH and BSA are commonly used as protein carriers for haptens such as peptides, in order to enable the immune response to these small molecules. KLH is most popular for immunization while BSA conjugate is used in immunoassays of the resulting antibodies in order to filter out the anti KLH response. The Maleimide activated KLH and BSA are preactivated with a heterobifunctional cross-linker (GMBS). These activated proteins can be reacted with compounds that contain a free sulfhydryl group to form a stable thioether bond. Maleimide groups per KLH molecule: 160-320 Coupling pH: 6.5-7.5
Form:
Each vial contains 5mg of Maleimide Activated KLH lyophilized from 10mM sodium phosphate buffer, pH 6.6, with 0.115mM NaCl, 1mM EDTA, and 40mM sucrose as stabilizer.
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