Purification Media, BioSeparation(TM) Affinity, Protein G
Biozol Catalog Number:
USB-P9100-80
Supplier Catalog Number:
P9100-80
Alternative Catalog Number:
USB-P9100-80-10,USB-P9100-80-50
Manufacturer:
US Biological
Category:
Molekularbiologie
Recombinant Protein G used also in Protein G beads (P9100-85) is a genetically engineered form of Protein G. Non-essential regions have been removed while leaving the IgG binding sites intact. Protein G has been extensively used for the isolation of IgG from several species of mammals. Although binding of Protein G to IgGs is not equivalent for all IgG subclasses, Protein G possesses properties that have made it a popular choice for antibody isolation. Protein G binds immunoglobulins at the Fc region of leaving the Fab region free to bind the antigen. Hence, Protein G is extremely useful for isolating of immune complexes. Source: Recombinant protein, expressed in E. coli Storage & Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with ddH2O, 0.1% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
Molecular Weight:
33
Purity:
90% by SDS-PAGE. Spectroscopic Analysis: OD280nm/250nm=2.51 Endotoxin: 0.5 EU/mg (LAL).
Form:
Supplied as a white lyophilized powder from PBS, pH 7.4. 10.6mg Recombinant Protein G, 0.78mg phosphate buffer salts. Reconstitute with 480ul of dH2O, 0.09% sodium azide.
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