Source: Staphylococcus aureus, Cowan I Strain Structure: Protein A is a monomeric protein lacking cysteine residues. Specificity: Protein A binds the Fc portion of human IgG1, IgG2, IgG3 (weakly), IgG4, and mouse IgG1 (weakly), IgG2a, IgG2b, and IgG3. Protein A also binds IgG from monkey, rabbit, porcine, guinea pig, canine, and feline. Applications: Suitable for use in ELISA, Western Blot, Affinity Chromatography, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 1:8000-1:16,000. However, for any specific experiment, the conjugate should be titrated to get the highest signal possible with lowest background. Optimal dilutions to be determined by the researcher. Substrate Solution: 1mM ABTS in 0.1M citrate buffer, pH 4.2, 0.03% hydrogen peroxide. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 6 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Purified by Chromatography.
Form:
Supplied as a liquid in PBS, pH 7.4, 1% ovalbumin, 40% glycerol, 0.1% proclin. Labeled with with highly purified horseradish peroxidase (HRP)(RZ > 3.0).
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