Native Protein G is a surface receptor from Streptococcus sp. which binds the Fc portion of immunoglobulins, binding 2 molecules of IgG. Applications: Suitable for use in Immunomicroscopy and FLISA. Other applications not tested. Recommended Dilutions: Immunomicroscopy: 1:500-1:2500 Optimal dilutions to be determined by the researcher. Reconstitution: Use 1ml of ddH2O. For extended storage reconstitute product with sterile 50% glycerol, ddH2O instead of water and then aliquot contents and freeze at -20C or lower. Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Chromatographically purified Protein G. Single band by IEP against anti-Fluorescein and anti-Protein G.
Form:
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% sodium azide. Labeled with Fluorescein isothiocyanate (FITC) (MW 390D).
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