Applications: Suitable for use in Immunoblot (Western or Dot Blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:10,000-1:40,000 is recommended for blotting rabbit IgG. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder is stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O prior to use. To avoid repeated freezing and thawing, aliquot reconstituted stock solution and store at -20C. Reconstituted product is stable for 12 months at -20C. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Protein G was purified by chromatography prior to conjugation.
Form:
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA. Labeled with horseradish peroxidase (HRP).
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