Protein Gene Product 9.5, Recombinant, Human (PGP9.5, Ubiquitin CT Hydrolase L1, UCH, UCHL1, Ubiquitin thiolesterase, PARK5)

Product Overview

• Article Name: Protein Gene Product 9.5, Recombinant, Human (PGP9.5, Ubiquitin CT Hydrolase L1, UCH, UCHL1, Ubiquitin thiolesterase, PARK5)
• Biozol Catalog Number: USB-P9102-70H
• Supplier Catalog Number: P9102-70H
• Alternative Catalog Number: USB-P9102-70H-100,USB-P9102-70H-500
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

PGP9.5/UCH-L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. PGP9.5 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. PGP9.5 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinsons disease susceptibility. Recombinant PGP9.5 protein was expressed in E.coli and purified by using conventional chromatography techniques. Molecular Weight: ~24.8kD (223 a.a) Amino Acid Sequence: MQLKPMEINP EMLNKVLSRL GVAGQWRFVD VLGLEEESLG SVPAPACALL LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA NNQDKLGFED GSVLKQFLSE TEKMSPEDRA KCFEKNEAIQ AAHDAVAQEG QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DTLLKDAAKV CREFTEREQG EVRFSAVALC KAA Enzyme Activity: > 150 pmol/min/ug, Unit Definition: efined as the amount of enzyme that hydrolysis 1.0 pmole of ubiquitin-AMC per minute at pH 7.5, at 37C. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 24.8
• Purity: 95% by SDS PAGE
• Form: Supplied as a liquid in 20mM Tris-HCl pH8.0, 2mM EDTA.