Protein Kinase G1 beta, N-terminal HIS-Tag, Recombinant, Human (PKG1 beta, Cyclic GMP-dependent Protein Kinase)
Biozol Catalog Number:
USB-P9102-95R
Supplier Catalog Number:
P9102-95R
Alternative Catalog Number:
USB-P9102-95R-10
Manufacturer:
US Biological
Category:
Molekularbiologie
Source: Recombinant corresponding to full length human PKG1 beta with N-terminal His tag expressed in Baculovirus infected Sf9 cell expression system (NM_006258). Specific Activity: 0.85pmoles/min/ug. Enzyme reaction is conducted in a buffer containing 50mM HEPES, pH 7.5, 10mM magnesium chloride, 1mM EGTA, 200uM ATP, 0.01% Brij-35, 2uM substrate (SER/THR 14) at RT for 1hour. Applications: Suitable for use in the study of enzyme kinetics, screening inhibitors and selectivity profiling. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing. Store at -70C. Aliquots are stable for at least 12 months at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
