Protein Kinase C gamma, Recombinant, Human, SDS Standard (PKCg)
Biozol Catalog Number:
USB-P9103-25J
Supplier Catalog Number:
P9103-25J
Alternative Catalog Number:
USB-P9103-25J-1
Manufacturer:
US Biological
Category:
Molekularbiologie
Protein Kinase C (PKC), one of the most important proteins in signal transduction is involved with cell proliferation, differentiation and function. PKC is a family of Ca++ phosphatidylserine dependent, serine/threonine kinases with 12 known isozyme members (a, bI, bII, g, d, e, z, h, q, l, m, i). PKC isozyme expression and distribution varies at both the tissue and intracellular levels. PKC is activated by diacylglycerol. PKC functions are dependent on isozyme type and location. PKC g (gamma, PKC type I) has a MW of 78kD. It is found predominantly in the brain and spinal cord. Recombinant protein corresonding to human kinase C g expressed in baculoviral insect expression system. Enzyme activity: Not enzymatically active. Applications: Suitable for use as a standard for Western blots. Other applications not tested. Recommended Dilutions: For Western blots using enhanced chemiluminescence, load 2-5ul of prepared standard directly from vial. No dilution is necessary. For blots using less sensitive substrates, such as alkaline phosphatase, larger amounts of protein should be loaded onto the gel. Optimum dilutions to be determined by researcher. Storage and Stability: Stable indefinitely at -70C and for at least one year at -20C.
Form:
Suspended in SDS/mercaptoethanol sample buffer with a bromophenol blue dye marker.
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