Protein Tyrosine Phosphatase 1B, full length, Recombinant, Human (PTP1B)

Product Overview

• Article Name: Protein Tyrosine Phosphatase 1B, full length, Recombinant, Human (PTP1B)
• Biozol Catalog Number: USB-P9109-01A
• Supplier Catalog Number: P9109-01A
• Alternative Catalog Number: USB-P9109-01A-20
• Manufacturer: US Biological
• Category: Molekularbiologie

Product Description

Full-length (aa1-436) PTP1B with N-terminal GST-tag (GenBank Accession No. M31724) Specific Activity: ~2,000 pmole/min/ug. One unit will hydrolyze 1nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30C Assay Buffer: 50mM Hepes, pH 7.4, 2mM EDTA, 3mM DTT, 100mM sodium chloride, 50mM pNPP. Applications: Suitable for use in enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors. Other applications not tested. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70C. Aliquots are stable for 6 months.For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Product Properties

• Molecular Weight: 76
• NCBI: 31724
• UniProt: P18031
• Purity: 55% by SDS-PAGE
• Form: Supplied as a liquid in 45mM Tris-HCl, pH 8.0,124 mM NaCl, 2.4mM KCl, 10% glycerol, 18mM glutathione, and 3mM DTT