Protein Tyrosine Phosphatase 1B, Recombinant, Human (PTP1B)
Biozol Catalog Number:
USB-P9109-14
Supplier Catalog Number:
P9109-14
Alternative Catalog Number:
USB-P9109-14-250
Manufacturer:
US Biological
Category:
Molekularbiologie
The protein coding region of the catalytic domain of PTP-1B (aa1-321) was cloned into an E. coli expression vector. Accession: NP_002818. Amno Acid Sequence: MEMEKEFEQI/ DKSGSWAAIY/ QDIRHEASDF/ PCRVAKLPKN/ KNRNRYRDVS/ PFDHSRIKLH/ QEDNDYINAS/ LIKMEEAQRS/ YILTQGPLPN/ TCGHFWEMVW/ EQKSRGVVML/ NRVMEKGSLK/ CAQYWPQKEE/ KEMIFEDTNL/ KLTLISEDIK/ SYYTVRQLEL/ ENLTTQETRE/ ILHFHYTTWP/ DFGVPESPAS/ FLNFLFKVRE/ SGSLSPEHGP/ VVVHCSAGIG/ RSGTFCLADT/ CLLLMDKRKD/ PSSVDIKKVL/ LEMRKFRMGL/ IQTADQLRFS/ YLAVIEGAKF/ IMGDSSVQDQ/ WKELSHEDLE/ PPPEHIPPPP/ RPPKRILEPH/ N Specific Acitivity: 6,000-12,000units/mg. Unit Definition: One unit will hydrolyze 1nanomole of p-nitrophenylphosphatate (p-NPP) per minute at pH 7.4, 37C using 10mM of substrate. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
